Keywords: Helicobacter pylori infection detection methods
Helicobacter pylori infection in check many ways, including direct examination of bacteria uremic enzyme activity assay, immunological detection and polymerase chain reaction method.
Direct examination: (1) bacteria by gastroscopy pliers to take the gastric mucosa (antral mucosa) for direct smear, stained tissue sections staining and bacterial culture to detect the HP. Gastric mucosal bacterial culture is the most reliable diagnostic HP can be used as the “gold standard" validation of other diagnostic tests, while susceptibility testing to guide clinical use of drugs.
(2) uremic enzyme check: because HP is a the human stomach unique able to produce large amounts of the uremic enzyme bacteria, it can be diagnosed by detecting uremic enzyme HP infection. Uremic enzymatic decomposition intragastric uremic generate ammonia and carbon dioxide to reduce the concentration of urea, ammonia concentration increased. A variety of detection methods have been developed based on this principle: test ① the gastric biopsy uremic enzyme; ② breath test; (3) gastric juice urea or urea nitrogen measurement; ④ 15N-urea test.
(3) immunological detection: There are a variety of immunological detection methods to detect HP infection by measuring the HP antibody in the serum, including complement fixation test, agglutination test, passive hemagglutination determination of the Western blot assay and enzyme joint (ELISA) and the like.
(4) polymerase chain reaction technology: normal gastric mucosa is rarely detected in HP (0 to 6%), patients with chronic gastritis HP high detection rate of approximately 50% to 80% of patients with chronic active gastritis HP detection the rate is even higher, more than 90%.