Key words nasopharyngeal tumor; transfer; α catenin; γ catenin; matrix metalloproteinase 7
Α cat, γ cat abnormal expression of a variety of tumors has been confirmed is closely related to tumor invasion and metastasis. MMP 7 the MMP family only by epithelial tumor cell-specific expression of the enzyme, is directly related to tumor metastasis . α cat, γ cat and MMP 7 in nasopharyngeal carcinoma and lymph node metastasis in the relationship, have not been reported.
1 Materials and methods
Collect Pathology, Affiliated Hospital of Guangdong Medical College from 2005 to 2006, archived data. The nasopharyngeal mucosa chronic inflammation 30 cases, differentiated non-keratinizing nasopharyngeal carcinoma, 30 cases of metastatic cervical lymph node metastasis in 20 cases. α cat, γ cat, MMP 7 antibody and SP immunohistochemical kit were purchased from Beijing Zhongshan.
SP kit manual step operation. A gastric positive piece as a positive control, PBS instead of primary antibody as a negative control.
1.3 The results of judgment
normal staining of α cat, γ cat that membrane brown color grading standards: 0, expression disappeared; 1 cytoplasmic expression; 2 points, heterogeneity in expression was decreased; 3 membrane expression. 3 is divided into normal expression. MMP 7 staining was mainly localized in the cytoplasm, cytoplasmic brownish yellow positive cells; ≥ 10% positive cells “+"; semi-quantitative grading: (-): <10%; (+): 10% to 75%。">25%; (+ +): 26% to 50%; (+ + +): 51% to 75%; (+ + +):> 75%.
1.4 statistical methods
Χ2 test, t test, and Speaman correlation analysis.
The 2.1 α cat, γ cat and MMP 7 expression in nasopharyngeal benign and malignant lesions
Mucosal chronic inflammation of α cat, γ cat abnormal expression rate was significantly lower than that nasopharyngeal carcinoma (χ2 = 38.79, 42.15) and lymph node metastasis (χ2 = 35.42, 34.90) (P <0.01). Nasopharyngeal MMP 7 positive expression rate was significantly higher than the mucosa of chronic inflammation (χ 2 = 9.61, P <0.01) and lymph node metastasis (χ 2 = 10.08, P <0.01), as shown in Table 1, Figure 1 to 3. Table 1 α cat, γ cat and of MMP 7 in nasopharyngeal benign and malignant lesions, expression of
2.2 α cat, γ cat dyed in nasopharyngeal benign and malignant lesions graded
Mucosal chronic inflammatory α cat, γ cat staining graded significantly higher than nasopharyngeal (t = 8.07, 8.76) and lymph node metastasis (t = 9.62, 18.26) (P <0.01); nasopharyngeal of γ cat in a significant higher than the lymph node metastasis (t = 2.11, P <0.05), see Table 2.
2.3 MMP 7 and of α cat, of γ cat in the relationship of nasopharyngeal cancer, lymph node metastasis
21 cases of MMP 7 (+) nasopharyngeal cancer, 19 cases of of α cat and 18 cases of γ cat abnormal expression of MMP 7 of α cat, γ cat no significant correlation (rs = -0.088, 0.268, P> 0.05). Lymph node metastasis in 20 cases, 4 cases of MMP 7 (+) of α cat and γ cat were abnormal expression; 16 cases of MMP 7 (-), 16 cases of of α cat and 15 cases of γ cat abnormal expression; MMP 7 of α cat, γ cat was a significant positive correlation (rs = 0.636, 0.55, P <0.01).
α cat E cad connect the necessary components of the cytoskeleton, whose table of α cat dyeing grading of γ cat in nasopharyngeal benign and malignant lesions lack, you can not form a stable cell connection. γ cat mediated E cad cat complex formation and the homogeneous adhesion plays an important role. expression of α cat down with gastric cardia poorly differentiated trends and metastatic potential related ; expression missing of γ cat in the hepatoma cell membrane, cytoplasm and nucleus and a large number of expression , and lymph node metastasis. . Mainly abnormal expression rate was significantly higher than the this study of α cat, γ . Mucosa with chronic inflammation in almost normal expression; nasopharyngeal cancer, lymph node metastasis by expression of deletion and expression sites change chronic inflammation of mucous membrane staining grading significant below the mucosa of chronic inflammation. Prompts the nasopharyngeal cells a cat, γ cat abnormal expression can be induced E cad / cat complex functional changes, reduced intercellular adhesion, cell easy transfer. Γ cat staining graded lymph node metastasis was significantly lower than that nasopharyngeal prompt γ cat expression abnormalities may be mediated tumor cell adhesion to reduce the role of more obvious. In this study, nasopharyngeal carcinoma and lymph node metastasis in the of α cat heterogeneity of expression weakened mainly γ cat loss of expression. Its significance: First, the NPC may exist the of α cat, and γ cat expression the exception of the different mechanisms; Second, α cat accumulate in the cytoplasm may induce tumor cell proliferation.
And MMP 7 said the matrilysin MMP 2 members can activate and inactivate serpins, and tumor metastasis-associated . MMP 7 in gastric high expression weakly expressed in cancer tissue, and lymph node metastasis was a significant correlation . In this study, nasopharyngeal MMP 7 positive rate was significantly higher than the chronic inflammation of mucous membranes; prompt tumor cells possibly through the secretion of MMP 7 degradation of extracellular matrix to promote nasopharyngeal carcinoma invasion and metastasis. MMP 7 low expression in lymph node metastasis, the reason may be promising nasopharyngeal cancer cells to form metastases in the lymph nodes, may occur evolution, accompanied by its heterogeneity change; cancer cells in the lymph node metastases further mechanism of transfer may be associated with different primary tumors.
NPC study no Catenins and MMP reported. In this study, MMP α cat, γ cat abnormal expression in lymph node metastases showed a significant positive correlation, while the nasopharyngeal no correlation. Combined with lymph node metastasis in MMP 7 expression situation, despite α cat, γ cat abnormal expression rate was significantly higher, but the expression of MMP 7 without a corresponding rise, suggesting that α cat, γ cat abnormal expression of MMP 7 nasopharyngeal carcinoma metastasis did not show a synergistic effect. For α cat, of γ cat and MMP 7 in the NPC relations need to be further explored.